2. Sedimentation activity test.


It provides a rapid evaluation of microfloral activity.

Must be conducted just after sample collection.



  1. Put a sample of rumen fluid in a test tube and let to stand.

  2. Measure the time needed for completion of sedimentation of fine particles and floatation of coarse solid particles

        Normal time is 4 - 8 minutes.

        Abnormal time

        Very rapid sedimentation with no floatation occurs in :-

o Rumen acidosis.

o Prolonged anorexia.

o Inactive microflora from indigestible roughages.

        No appreciable sedimentation or floatation :-

o Frothy bloat.

o Some cases of vagal indigestion.


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